DNA sequences and plasmids for the preparation of sugar beet with changed sucrose concentration

ABSTRACT

PCT No. PCT/EP94/01671 Sec. 371 Date Nov. 17, 1995 Sec. 102(e) Date Nov. 17, 1995 PCT Filed May 20, 1994 PCT Pub. No. WO94/28146 PCT Pub. Date Dec. 8, 1994DNA sequences and plasmids are described, that by integration in a plant genome of sugar beet change the sucrose concentration. In addition, transgenic plants that, by introduction of the DNA sequences of the invention, have altered sugar concentrations, are described.

FIELD OF THE INVENTION

The present invention relates to DNA sequences and plasmids, containing these DNA sequences, which by integration into the genome of a sugar-beet plant, changes the sugar metabolism of the plant to be changed. The invention also relates to transgenic plants formed with the help of these sequences.

BACKGROUND OF THE INVENTION

Sucrose is of central importance for the plant and serves many functions. For the long distance transport of photoassimilates and/or energy between various organs in plants, sucrose is almost exclusively used. The sucrose, which is transported in a specific heterotrophic organ, determines the growth and the development of this organ. Thus it is known, e.g. from EP 442 592, that transgenic plants, in which the transport away of the sucrose from the exporting leaves is inhibited by expression of an apoplastic invertase, shows a strong reduction in the growth of e.g. roots or tubers in the case of potato plants. For tobacco plants, the principal importance of sucrose as the central function for the long distance transport of energy carriers within the plant is described (von Schaewen et al, 1990, EMBO J 9: 3033-3044).

Further it is also known from EP 455 316 that DNA sequences present on plasmids, after introduction in a plant genome of a potato plant can affect the starch biosynthesis as well altering the amount and composition of the protein in the potato tubers.

While it has known that a reduction of the amount of sucrose imported in the heterotrophic organs, such as tubers and seeds, leads to loss of yield, it is not known whether an increase in the amount of sucrose in the photosynthetically active parts of the plant, mainly the leaves, leads to a better supply of heterotrophic organs and thus to an increase in yield.

Besides sucrose and/or the hexoses, glucose and fructose, derived from sucrose, have the property of protection of plants against frost damage at low temperatures. Frost damage is one of the main limiting factors in agricultural productivity in the northern hemisphere. Temperatures below freezing lead to the formation of ice crystals. Since the growing ice crystals consist of pure water, water is extracted from the cells as the temperature falls.

This dehydration has at least two potential damaging results:

1. All dissolved substances within a cell are strongly concentrated and the cell contracts following the loss of water. Highly concentrated salts and organic acids lead to membrane damage.

2. With rehydration from dew, the previously contracted cells reexpand. The cell membrane also expands again. The volume expansion puts a heavy mechanical load on the membrane.

It is thus clear that a freezing/dew cycle can lead to severe membrane damage of the cells and thus to damage to the plant.

SUMMARY OF THE INVENTION

It thus appears worth trying to hinder the freezing. One possible strategy is the increased formation of osmotically active substances in the cytosol of plant cells. This should lead to a lowering of the freezing point. Osmotically active substances include sucrose and/or the two hexoses derived from sucrose.

The increased formation of sucrose and/or the two hexoses at low temperatures is desirable in the growing plant. Another situation can exist in the harvested parts of a plant, especially in storage.

In relation to the economic aspects, sucrose thus possesses two especially important functions:

1 as the transport form for the distant transport of photoassimilates,

2 as an osmotically active substance with the desirable activity of lowering the freezing point in intact, growing plants.

The biosynthesis pathways for the formation of sucrose, either from the primary photosynthesis products (in the leaf) or by breakdown of starch (in the storage organs e.g. of potatoes), are known.

It is however, not known how and in what way changes of the carbohydrate concentration in sugar beet can be achieved since it is not possible to use even very similar genes, such as for example genes that code for a sucrose synthase, ADP-glucose pyrophosphorylase or sucrose phosphate synthase of the potato with satisfactory success for the preparation of sugar beet with changed sucrose concentration. An exact analysis and determination of the DNA sequences or sequence fragments for the sugar beet is thus required.

To change the sugar concentration in sugar beet, DNA sequences are now provided which code for the small and large subunit of the ADP glucose pyrophosphorylase, the sucrose synthase and the sucrose phosphate synthase of sugar beet (Seq. ID No 1-8).

DETAILED DESCRIPTION OF THE INVENTION

The DNA sequences (Seq. ID No. 1-4) can be introduced into plasmids and in this way combined with steering elements for expression in eukaryotic cells. Such steering elements are, on the one hand, transcription promoters and, on the other hand, transcription terminators. Each plasmid comprises:

a) a suitable promoter which ensures that the coding sequence meets a suitable time point or in the specified developments in the transgenic plant or in determined genes of transgenic plants;

b) at least a coding sequence for sugar beet that

i) is so coupled to the promoter that the formation of an RNA is allowed which is into a protein, whereby the protein demonstrates an enzymatic activity which leads to a change of the sucrose concentration in the plant, or

ii) which is so coupled to the promoter that the coding part is read, which leads to the formation of a so-called anti-sense RNA which under-expresses the formation of the protein coded from an endogenous gene in the plant, that is involved in the sucrose biosynthesis; and

c) A non-coding termination sequence that contains the signal for the termination and polyadenylation of the transcript.

The coding sequences named under b) are the sequences that code for the large and small subunit of the ADP glucose pyrophosphorylase, for the sucrose phosphate synthase and for the sucrose synthase of sugar beet.

The large subunit of the ADP-glucose-pyrophosphorylase has following nucleotide sequence (Seq. ID Nos. 1 and 2): ##STR1##

The small subunit of the ADP-glucose-pyrophosphalase has the following nucleotide sequence (Seq. ID Nos. 3 and 4): ##STR2##

The sucrose phosphate--synthase has the following nucleotide sequence (Seq. ID Nos. 5 and 6): ##STR3##

The sucrose-synthase has the following nucleotide sequence (Seq. ID Nos. 7 and 8): ##STR4##

These sequences can also be combined together in a suitable plasmid which leads to a combination of the individual characteristics, conditioned by the expression of the protein.

The promoter should ensure that the foreign gene is expressed in the plant. The promoter can be so chosen that the expression occurs only in specified tissues, at a determined time point in the plant's development or at a time point determined by outside influences. The promoter can be homologous or heterologous to the plant. Suitable promoters are e.g. the promoter of the 35S RNA of the cauliflower mosaic virus, the patatin promoter B33 (Rocha-Sosa et al. (1989) EMBO J 8: 23-29) or a promoter that ensures an expression only in photosynthetically active tissues. Other promoters can be used which ensure expression only in specified organs, such as the root, tuber, seed, stem or specified cell types such as mesophyllic, epidermal or transport cells.

The coding sequences described herein contain the information for the formation of an mRNA for the large subunit of the ADP-glucose-pyrophosphorylase and the sucrose-phosphate-synthase (SPS) and a part of the information for formation of the small subunit of the ADP-glucose-pyrophosphorylase as well as the sucrose-synthase, that are suitable for the formation of anti-sense RNA to the corresponding genes. Whether a translatable mRNA or an anti-sense nucleic acid is formed, depends on the orientation of the coding sequence in relation to the promoter. If the 3' end of the coding sequence is fused to the 3' end of the promoter, an anti-sense RNA results, while fusion of the 5' end of the coding to the 3' end of the promoter produces a translatable RNA. The latter leads to an increase of the enzyme activity in the cell, while the former leads to a reduction of the enzyme activity in the cell.

The coding sequence for the large and small subunit of the ADP-glucose-pyrophosphorylase, the sucrose phosphate synthase and the sucrose synthase can be one of those described in this invention or can be one that is derived by modifications of the sequences described above. Thereby especially modifications of the sequences can be considered which lead to by-passing of the plant's own regulation mechanisms. Modifications to the DNA sequences of the invention can be by known methods, such as e.g. base exchange or targeted or non-targeted mutagenesis. The so-formed derivatives of the DNA sequences of the invention are also within the scope of the invention.

With plasmids, which contain one or more of the DNA sequences of the invention, sugar beet can be transformed with the object of raising and/or reducing the enzyme activity and/or the change of the sucrose concentration.

For the introduction of the DNA sequences of the invention in sugar beet, a large number of cloning vectors are available, which contain a replication signal for E. coli and a marker, which allows a selection of the transformed cells.

According to the introduction method of the desired gene in the plant, other DNA sequences may be suitable. Should the Ti- or Ri-plasnid be used, e.g. for the transformation of the plant cell, then at least the right boundary, often however, both the right and left boundary of the Ti- and Ri-Plasmid T-DNA, is attached, as a flanking region, to the gene being introduced. The use of T-DNA for the transformation of plants cells has been intensively researched and is well described in EP 120 516; Hoekama, In: The Binary Plant Vector System, Offset-drukkerij Kanters B. V. Alblasserdam, (1985), Chapter V; Fraley, et al., Crit. Rev. Plant Sci., 4:1-46 and An et al. (1985) EMBO J. 4: 277-287. Once the introduced DNA is integrated in the genome, it is generally stable there and remains also in the offspring of the original transformed cells. It normally contains a selection marker, which induces resistance in the transformed plant cells against a biocide or antibiotic such as kanamycin, G 418, bleomycin, hygromycin or phosphinotricin etc. The individual marker employed should therefore allow the selection of transformed cells from cells, which lack the introduced DNA.

For the introduction of DNA into a plant, besides transformation using Agrobacteria, there are many other techniques available. These techniques include the fusion of protoplasts, microinjection of DNA and electroporation, as well as ballistic methods and virus infection. From the transformed plant material, whole plants can be regenerated in a suitable medium, which contains antibiotics or biocides for the selection. The resulting plants can then be tested for the presence of introduced DNA. No special demands are placed on the plasmids in injection and electroporation. Simple plasmids, such as e.g. pUC-derivatives can be used. Should however whole plants be regenerated from such transformed cells the presence of a selectable marker gene is necessary. The transformed cells grow within the plants in the usual manner (see also McCormick et al.(1986) Plant Cell Reports 5: 81-84). These plants can be grown normally and crossed with plants, that possess the same transformed genes or different genes. The resulting hybrid individuals have the corresponding phenotypical properties.

In order to understand the examples forming the basis of this invention all the processes necessary for these tests and which are known per se will first of all be listed:

1. Cloning process

The vectors pUC 18/19 and M13mp10 series (Yanisch-Perron et al. (1985) Gene 33: 103-119), as well as the vector EMBL 3 (Frischauf et al. (1983) J Mol Biol 170: 827-842) were used for cloning.

For the plant transformations, the gene constructs were cloned in the binary vector BIN 19 (Bevan (1984) Nucl. Acids Res 12: 8711-8720)

2. Bacterial strains

The E. coli strain BMH71-18 (Messing et al., Proc. Natl. Acad. Sci. USA (1977), 24, 6342-6346) or TB1 was used for the pUC and M13 mP vectors.

For the vector BIN19, the E. coli strain TB1 exclusively, was used. TB1 is a recombinant-negative, tetracycline-resistant derivative of strain JM101 (Yanisch-Perron et al., Gene (1985), 33, 103-119). The genotype of the TB1 strain is (Bart Barrel, personal communication): F' (traD36, proAB, lacI, lacZΔM15), Δ(lac, pro), SupE, this, recA, Sr1::Tn10(TcR).

The transformation of the plasmids into the potato plants was carried out using Agrobacterium tumefaciens strain LBA4404 (Bevan, (1934), Nucl. Acids Res. 12, 8711-8720).

TransporTation of Agrobacterium tumefaciens

In the case of BIN19 derivatives, the insertion of the DNA into the Agrobacterium was effected by direct transformation in accordance with the method of Holsters et al., (1978) (Mol Gene Genet 163: 181-187). The plasmid DNA of the transformed Agrobacterium was isolated in accordance with the method of Birnboim and Doly (1979) (Nucl Acids Res 7: 1513-1523) and was analysed by gel electrophoresis after suitable restriction cleavage.

4. Sucrose phosphate-synthase activity test

The sucrose phosphate-synthase activity was determined according to the method of Siegel and Stitt (1990, Plant Science 66: 205-210) in a two stage analysis. To 180 μl of a solution of 50 mM HEPES/KOH (pH 7.4), 5 mM magnesium chloride, 5 mM fructose-6-phosphate, 25 mM glucose-6-phosphate and 6 mM uridine-5'-diphosphoglucose, 20 μl of probe was added and incubated for 10 minutes at 25° C. It was heated for 3 minutes at 95° C., to complete the reaction. After centrifuging, the supernatant was spectroscopically analysed for the liberation of uridine-5'-diphosphate, whereby a pyruvate-kinase coupling enzyme reaction was used. Preparations without hexose phosphate, as well as the measurement of the recovery of added uridine-5'-diphosphate act as controls.

EXAMPLES Example 1

Cloning of cDNA to large and small subunits of the ADP glucose pyrophosphorylase of sugar beet.

From the storage roots of 3-4 month old sugar beet plants grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Biochem 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an XhoI recognising position and for synthesis of the first cDNA strand, methylated cytidine nucleotide was inserted. After synthesis of the two strands, an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease XhoI. In this way the hemimethylation of cDNAwas hindered so that an internal XhoI recognition position is cut. By theseprocedures there is obtained a population of cDNA molecules that can be cloned directly into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with the total cDNA fragment of the large and/or subunit of the AGPase of potato (Muller-Roeber et al., 1990, MGG 224, 136-146) as an EcoRI-fragment. The recombinant phages corresponding to thehybridising signal were isolated. By in vivo excision, plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions, individual clones were analysed by determination of theprimary sequence.

Example 2

Cloning of cDNA to sucrose-phosphate-synthase (SPS) from sugar beet.

From the storage roots of 3-4 month old sugar beet plant grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Biochem 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an XhoI recognising position and for synthesis of the first cDNA strand, methylated cytidine nucleotide was inserted. After synthesis of the two strands, an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease XhoI. In this way the hemimethylation of cDNAwas hindered so that an internal XhoI recognition position is cut. By theseprocedures there is obtained a population of cDNA molecules that can be cloned directly into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with the total cDNA fragment of the sucrose-phosphate-synthase (SPS) from spinach (Sonnewald, 1992, Planta) asNotI. The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision, plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions, individual clones were analysed by determination of the primary sequence.

Example 3

Cloning of cDNA to sucrose-synthase from sugar beet.

From the storage roots of 3-4 month old sugar beet plants grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Biochem 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an XhoI recognising position and for synthesis of the first cDNA strand, methylated cytidine nucleotide was inserted. After synthesis of the two strands, an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease XhoI. In this way the hemimethylation of cDNAwas hindered so that an internal XhoI recognition position is cut. By theseprocedures there is obtained a population of cDNA molecules that can be cloned directly into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with both EcoRI/BgIII sub fragments sucrose synthase from maize (Worrell et al., 1991, Plant Cell 3, 1121-1130). The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision, plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in thebacteria. After checking the size of the insertions, individual clones wereanalysed by determination of the primary sequence.

Example 4

Determination of the nuclectide sequence of the ADP glucose pyrophosphorylase, the sucrose synthase and the sucrose phosohate synthaseof sugar beet and derivation of the corresponding amino acid sequences

The nucleotide sequences of the instructions obtained from Examples 1-3, were determined by standard methods by means of the dideoxy method (Sangeret al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467). The nucleotide sequences and the amino acid sequences derived therefrom are given in the sequence protocols Seq. ID No. 1-4.

The sequences are shown earlier; the protocols are as follows:

SEO ID NO: 1

SEQUENCE TYPE: Nucleotide with corresponding protein

SEQUENCE LENGTH: 1924 base pairs

STRANDEDNESS: single

TOPOLOGY: linear

MOLECULE TYPE: cDNA

ORIGINAL SOURCE

ORGANISM: Beta vulgaris

IMMEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap

FEATURES: from 206 to 1770 coding region

PROPERTIES: ADP-glucose-pyrophosphorylase, large subunit

SEO ID NO: 3

SEQUENCE TYPE: Nucleotide with corresponding protein

SEQUENCE LENGTH: 1763 base pairs

STRANDEDNESS: single

TOPOLOGY: linear

MOLECULE TYPE: cDNA

ORIGINAL SOURCE

ORGANISM: Beta vulgaris

IMMEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap

FEATURES: from 3 to 1469 coding region

PROPERTIES: ADP-glucose-pyrophosphorylase, small subunit

SEO ID NO: 5

SEQUENCE TYPE: Nucleotide with corresponding protein

SEQUENCE LENGTH: 3635 base pairs

STRANDEDNESS: single

TOPOLOGY: linear

MOLECULE TYPE: cDNA

ORIGINAL SOURCE

ORGANISM: Beta vulgaris

IMMIEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap

FEATURES: from 31 to 3164 coding region

PROPERTIES: Sucrose-phosphate-synthase

SEO ID NO: 7

SEQUENCE TYPE: Nucleotide with corresponding protein

SEQUENCE LENGTH: 2563 base pairs

STRANDEDNESS: single

TOPOLOGY: linear

MOLECULE TYPE: cDNA

ORIGINAL SOURCE

ORGANISM: Beta vulgaris

IMMMEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap

FEATURES: from 3 to 2300 coding region

PROPERTIES: Sucrose synthase

    __________________________________________________________________________     SEQUENCE LISTING     (1) GENERAL INFORMATION:     (iii) NUMBER OF SEQUENCES: 8     (2) INFORMATION FOR SEQ ID NO:1:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 1925 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: cDNA     (vi) ORIGINAL SOURCE:     (A) ORGANISM: Beta vulgaris     (C) INDIVIDUAL ISOLATE: ADP Glucose Pyrophosphorylase, large     subunit     (vii) IMMEDIATE SOURCE:     (A) LIBRARY: phage lamda zap     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 206..1774     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:     CAAAAGAAAAACTTCCCATTTCTACTTCTTTGCACAATATAATTTCCCACCAATTTTTCT60     TTAAATTTCTCACTTTCATTTAATCAGTTTTCAGCAACATTCTGATACTCGACAACCCAC120     TTTCTGTTCTCCCAAGATTCCAAACCTCTGATTCTCATTCCACTAATATTTTTGCTTATT180     TTTTTTCTGGATTTAAAGAAAAGCTATGGATGCAAGTGCAGCAGCCATAAAT232     MetAspAlaSerAlaAlaAlaIleAsn     15     GTCAATGCCCATTTAACAGAAGTTGGAAAGAAACGTTTTTTAGGAGAG280     ValAsnAlaHisLeuThrGluValGlyLysLysArgPheLeuGlyGlu     10152025     AGAATCAGTCAAAGTTTGAAGGGTAAAGATCTGAGAGCTCTGTTTTCA328     ArgIleSerGlnSerLeuLysGlyLysAspLeuArgAlaLeuPheSer     303540     AGAACTGAGAGCAAGGGTAGAAATGTCAATAAACCTGGGGTTGCATTT376     ArgThrGluSerLysGlyArgAsnValAsnLysProGlyValAlaPhe     455055     TCTGTTCTCACCTCAGATTTTAATCAAAGTGTTAAAGAATCTTTGAAA424     SerValLeuThrSerAspPheAsnGlnSerValLysGluSerLeuLys     606570     TATGAGCCAGCATTATTTGAATCTCCAAAAGCTGACCCAAAAAATGTG472     TyrGluProAlaLeuPheGluSerProLysAlaAspProLysAsnVal     758085     GCTGCAATTGTGCTGGGTGGTGGTGCTGGGACTCGCCTCTTTCCTCTT520     AlaAlaIleValLeuGlyGlyGlyAlaGlyThrArgLeuPheProLeu     9095100105     ACTAGCAGGAGAGCTAAGCCAGCAGTGCCAATTGGAGGGTGTTACAGG568     ThrSerArgArgAlaLysProAlaValProIleGlyGlyCysTyrArg     110115120     CTGATTGATGTGCCTATGAGCAACTGCATCAACAGTGGCATTAGAAAG616     LeuIleAspValProMetSerAsnCysIleAsnSerGlyIleArgLys     125130135     ATTTTCATTCTTACCCAGTTCAATTCGTTTTCGCTTAATCGTCATCTT664     IlePheIleLeuThrGlnPheAsnSerPheSerLeuAsnArgHisLeu     140145150     GCTCGAACCTATAATTTTGGAGATGGTGTGAATTTTGGGGATGGCTTT712     AlaArgThrTyrAsnPheGlyAspGlyValAsnPheGlyAspGlyPhe     155160165     GTGGAGGTTTTTGCTGCTACACAAACACCTGGAGAATCAGGAAAGAAA760     ValGluValPheAlaAlaThrGlnThrProGlyGluSerGlyLysLys     170175180185     TGGTTCCAGGGCACCGCTGATGCAGTAAGACAGTTTTTCTGGGCATTT808     TrpPheGlnGlyThrAlaAspAlaValArgGlnPhePheTrpAlaPhe     190195200     GAGGATTCCAAATCCAAGGATGTCGAGCATATAGTTATTTTATCCGGT856     GluAspSerLysSerLysAspValGluHisIleValIleLeuSerGly     205210215     GATCATCTTTACCGAATGGATTACATGAGTTTTTGGCAGAAGCACATT904     AspHisLeuTyrArgMetAspTyrMetSerPheTrpGlnLysHisIle     220225230     GACACCAATGCTGATATTACAGTGTCATGCATACCCATGGATGACAGC952     AspThrAsnAlaAspIleThrValSerCysIleProMetAspAspSer     235240245     CGTGCATCGGATTATGGGCTGATGAAGATTGATCACACTGGACGCATT1000     ArgAlaSerAspTyrGlyLeuMetLysIleAspHisThrGlyArgIle     250255260265     GTCCATTTTGCAGAAAAACCCAAGGGTTCTGATCTAACAGCAATGCAA1048     ValHisPheAlaGluLysProLysGlySerAspLeuThrAlaMetGln     270275280     GTAGATACAACTGTTCTTGGGCTCTCTGACCTTGAAGCTATGTCAAAT1096     ValAspThrThrValLeuGlyLeuSerAspLeuGluAlaMetSerAsn     285290295     CCATATATTGCATCAATGGGTGTTTATGTCTTTCGAACGGATGTTCTT1144     ProTyrIleAlaSerMetGlyValTyrValPheArgThrAspValLeu     300305310     ATGGAGCTTCTCAATCGAAAATACCCTTCAAGCAATGATTTTGGCTCT1192     MetGluLeuLeuAsnArgLysTyrProSerSerAsnAspPheGlySer     315320325     GAGATTATTCCTTCAGCTGTAGGAGAGTCTAATGTTCAGGCATATCTA1240     GluIleIleProSerAlaValGlyGluSerAsnValGlnAlaTyrLeu     330335340345     TTTAATGACTACTGGGAGGATATCGGAACCATAAAGTCTTTCTTTGAT1288     PheAsnAspTyrTrpGluAspIleGlyThrIleLysSerPhePheAsp     350355360     TCCAATTTGGCCCTTACACAACAGCCTCCCAAGTTTGAATTCTACGAT1336     SerAsnLeuAlaLeuThrGlnGlnProProLysPheGluPheTyrAsp     365370375     CCAAAAACACCTTTTTATACATCTGCAAGATTTCTGCCTCCTACAAAA1384     ProLysThrProPheTyrThrSerAlaArgPheLeuProProThrLys     380385390     GTCGACAGGTGCAAGATTGTCGATTCCATTGTATCCCATGGTTGTTTT1432     ValAspArgCysLysIleValAspSerIleValSerHisGlyCysPhe     395400405     CTACAGGAGTCTAGCATCCAACATTCCATTGTTGGTGTTCGCTCAAGA1480     LeuGlnGluSerSerIleGlnHisSerIleValGlyValArgSerArg     410415420425     TTAGAGTCCGGGGTTGAGTTCCAGGACACCATGATGATGGGCGCAGAT1528     LeuGluSerGlyValGluPheGlnAspThrMetMetMetGlyAlaAsp     430435440     TACTATCAAACTGAATCAGAAATTGCTTCTCTGCTTGCTGAGGGAAAG1576     TyrTyrGlnThrGluSerGluIleAlaSerLeuLeuAlaGluGlyLys     445450455     GTTCCTGTTGGTGTCGGACAGAATACCAAAATAAAGAATTGCATAATT1624     ValProValGlyValGlyGlnAsnThrLysIleLysAsnCysIleIle     460465470     GACAAGAACGCCAAAATTGGAAAAGATGTGGTAATCGCAAACACGGAT1672     AspLysAsnAlaLysIleGlyLysAspValValIleAlaAsnThrAsp     475480485     GGTGTTGAGGAAGCAGATAGACCAAATGAAGGCTTTTACATCAGGTCG1720     GlyValGluGluAlaAspArgProAsnGluGlyPheTyrIleArgSer     490495500505     GGCATTACCATCATTTTGAAGAACGCAACCATACAAGACGGTCTTGTG1768     GlyIleThrIleIleLeuLysAsnAlaThrIleGlnAspGlyLeuVal     510515520     ATTTAGATTTAATCATAACCTCATTAGAAAGAAATAATTTTGCATGATTTCCTTTT1824     Ile*     CATGTAACCTAAACTGGCTAAACCACGAGGTTTTCTCATCTGTATATATAATATGTCTAT1884     AACTATGGATAATCTTAATAAAAAAAAAAAAAAAAAAAAAA1925     (2) INFORMATION FOR SEQ ID NO:2:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 522 amino acids     (B) TYPE: amino acid     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: protein     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:     MetAspAlaSerAlaAlaAlaIleAsnValAsnAlaHisLeuThrGlu     151015     ValGlyLysLysArgPheLeuGlyGluArgIleSerGlnSerLeuLys     202530     GlyLysAspLeuArgAlaLeuPheSerArgThrGluSerLysGlyArg     354045     AsnValAsnLysProGlyValAlaPheSerValLeuThrSerAspPhe     505560     AsnGlnSerValLysGluSerLeuLysTyrGluProAlaLeuPheGlu     65707580     SerProLysAlaAspProLysAsnValAlaAlaIleValLeuGlyGly     859095     GlyAlaGlyThrArgLeuPheProLeuThrSerArgArgAlaLysPro     100105110     AlaValProIleGlyGlyCysTyrArgLeuIleAspValProMetSer     115120125     AsnCysIleAsnSerGlyIleArgLysIlePheIleLeuThrGlnPhe     130135140     AsnSerPheSerLeuAsnArgHisLeuAlaArgThrTyrAsnPheGly     145150155160     AspGlyValAsnPheGlyAspGlyPheValGluValPheAlaAlaThr     165170175     GlnThrProGlyGluSerGlyLysLysTrpPheGlnGlyThrAlaAsp     180185190     AlaValArgGlnPhePheTrpAlaPheGluAspSerLysSerLysAsp     195200205     ValGluHisIleValIleLeuSerGlyAspHisLeuTyrArgMetAsp     210215220     TyrMetSerPheTrpGlnLysHisIleAspThrAsnAlaAspIleThr     225230235240     ValSerCysIleProMetAspAspSerArgAlaSerAspTyrGlyLeu     245250255     MetLysIleAspHisThrGlyArgIleValHisPheAlaGluLysPro     260265270     LysGlySerAspLeuThrAlaMetGlnValAspThrThrValLeuGly     275280285     LeuSerAspLeuGluAlaMetSerAsnProTyrIleAlaSerMetGly     290295300     ValTyrValPheArgThrAspValLeuMetGluLeuLeuAsnArgLys     305310315320     TyrProSerSerAsnAspPheGlySerGluIleIleProSerAlaVal     325330335     GlyGluSerAsnValGlnAlaTyrLeuPheAsnAspTyrTrpGluAsp     340345350     IleGlyThrIleLysSerPhePheAspSerAsnLeuAlaLeuThrGln     355360365     GlnProProLysPheGluPheTyrAspProLysThrProPheTyrThr     370375380     SerAlaArgPheLeuProProThrLysValAspArgCysLysIleVal     385390395400     AspSerIleValSerHisGlyCysPheLeuGlnGluSerSerIleGln     405410415     HisSerIleValGlyValArgSerArgLeuGluSerGlyValGluPhe     420425430     GlnAspThrMetMetMetGlyAlaAspTyrTyrGlnThrGluSerGlu     435440445     IleAlaSerLeuLeuAlaGluGlyLysValProValGlyValGlyGln     450455460     AsnThrLysIleLysAsnCysIleIleAspLysAsnAlaLysIleGly     465470475480     LysAspValValIleAlaAsnThrAspGlyValGluGluAlaAspArg     485490495     ProAsnGluGlyPheTyrIleArgSerGlyIleThrIleIleLeuLys     500505510     AsnAlaThrIleGlnAspGlyLeuValIle     515520     (2) INFORMATION FOR SEQ ID NO:3:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 1763 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: cDNA     (vi) ORIGINAL SOURCE:     (A) ORGANISM: Beta vulgaris     (C) INDIVIDUAL ISOLATE: ADP Glucose Pyrophosphorylase, small     subunit     (vii) IMMEDIATE SOURCE:     (A) LIBRARY: phage lamda zap     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 3..1472     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:     GGATAACTGTGCCATCAACCTCCTCAAAGAACCTCCAAAATAGCCTC47     IleThrValProSerThrSerSerLysAsnLeuGlnAsnSerLeu     151015     GCATTCTCCTCTTCTTCTCTCTCCGGCGACAAAATTCAAACGACGTCA95     AlaPheSerSerSerSerLeuSerGlyAspLysIleGlnThrThrSer     202530     TTTCTCAACCGCCGATATTGTAGAATCTCTTCTAGAGCTCCGATTGTT143     PheLeuAsnArgArgTyrCysArgIleSerSerArgAlaProIleVal     354045     GTCTCTCCCAAAGCTGTTTCTGATTCTAAGAATTCGCAGACTTGTCTT191     ValSerProLysAlaValSerAspSerLysAsnSerGlnThrCysLeu     505560     GACCCTGAAGCCAGCCGTAGTGTTCTTGGTATTATACTTGGAGGTGGT239     AspProGluAlaSerArgSerValLeuGlyIleIleLeuGlyGlyGly     657075     GCTGGTACACGTCTTTACCCGTTGACTAAGAAGAGAGCCAAACCAGCC287     AlaGlyThrArgLeuTyrProLeuThrLysLysArgAlaLysProAla     80859095     GTGCCACTCGGTGCTAATTATAGGCTTATTGATATCCCAGTGAGCAAT335     ValProLeuGlyAlaAsnTyrArgLeuIleAspIleProValSerAsn     100105110     TGTTTGAACAGTAATATTTCCAAAATATATGTTCTTACACAATTCAAT383     CysLeuAsnSerAsnIleSerLysIleTyrValLeuThrGlnPheAsn     115120125     TCTGCTTCTCTGAATCGTCATCTTTCGCGGGCATATGCTAGCAACATG431     SerAlaSerLeuAsnArgHisLeuSerArgAlaTyrAlaSerAsnMet     130135140     GGAGGATACAAAAATGAGGGGTTTGTAGAAGTTCTTGCTGCTCAGCAA479     GlyGlyTyrLysAsnGluGlyPheValGluValLeuAlaAlaGlnGln     145150155     AGTCCAGAGAATCCAAACTGGTTTCAGGGTACAGCTGATGCTGTTAGG527     SerProGluAsnProAsnTrpPheGlnGlyThrAlaAspAlaValArg     160165170175     CAATATCTGTGGCTTTTCGAAGAGCACAATGTTCTTGAGTACTTGATT575     GlnTyrLeuTrpLeuPheGluGluHisAsnValLeuGluTyrLeuIle     180185190     CTTGCTGGTGACCATTTGTATCGAATGGATTATGAAAGATTTGTCCAA623     LeuAlaGlyAspHisLeuTyrArgMetAspTyrGluArgPheValGln     195200205     GCTCACAGAGAAACTGATGCAGACATTACTGTTGCTGCATTGCCAATG671     AlaHisArgGluThrAspAlaAspIleThrValAlaAlaLeuProMet     210215220     GATGAAAAGCGTGCTACTGCATTTGGTTTGATGAAAATTGATGAAGAA719     AspGluLysArgAlaThrAlaPheGlyLeuMetLysIleAspGluGlu     225230235     GGAAGAATTATTGAGTTTGCCGAGAAACCGAAAGGAGAACAATTGAAA767     GlyArgIleIleGluPheAlaGluLysProLysGlyGluGlnLeuLys     240245250255     GCTATGAAGGTTGATACCACAATCCTGGGTCTGGACGATGAGAGAGCA815     AlaMetLysValAspThrThrIleLeuGlyLeuAspAspGluArgAla     260265270     AAAGAAATGCCATTCATAGCCAGCATGGGCATATATGTTATTAGCAAA863     LysGluMetProPheIleAlaSerMetGlyIleTyrValIleSerLys     275280285     GATGTAATGCTTAATCTGCTTCGGGAGCAATTTCCTGGTGCTAATGAT911     AspValMetLeuAsnLeuLeuArgGluGlnPheProGlyAlaAsnAsp     290295300     TTTGGAAGTGAAGTTATTCCAGGCGCCACTTCCATAGGGTTGAGAGTC959     PheGlySerGluValIleProGlyAlaThrSerIleGlyLeuArgVal     305310315     CAAGCTTATTTGTATGATGGTTACTGGGAGGATATTGGTACCATTGAA1007     GlnAlaTyrLeuTyrAspGlyTyrTrpGluAspIleGlyThrIleGlu     320325330335     GCTTTTTACAATGCTAACTTGGGAATCACCAAAAAGCCGGTGCCAGAT1055     AlaPheTyrAsnAlaAsnLeuGlyIleThrLysLysProValProAsp     340345350     TTTAGCTTCTATGATCGTTCATCTCCAATTTATACACAACCTCGGTAT1103     PheSerPheTyrAspArgSerSerProIleTyrThrGlnProArgTyr     355360365     TTGCCTCCTTCAAAGATGCTTGATGCTGATATAACTGACAGCGTCATC1151     LeuProProSerLysMetLeuAspAlaAspIleThrAspSerValIle     370375380     GGTGAAGGCTGTGTTATTAAGAACTGTAAGATTCATCATTCTGTTATC1199     GlyGluGlyCysValIleLysAsnCysLysIleHisHisSerValIle     385390395     GGACTTCGATCTTGTATCTCGGAGGGTGCAATCATTGAGGACACACTG1247     GlyLeuArgSerCysIleSerGluGlyAlaIleIleGluAspThrLeu     400405410415     TTGATGGGAGCTGATTATTATGAGACTGATGCTGATCGGAAATTCCTG1295     LeuMetGlyAlaAspTyrTyrGluThrAspAlaAspArgLysPheLeu     420425430     GCTGCTAAGGGTAGTGTACCTATTGGAATTGGGAATGCACGTATTGGG1343     AlaAlaLysGlySerValProIleGlyIleGlyAsnAlaArgIleGly     435440445     GATGATGTCAAGATTATCAACAGTGACAATGTACAAGAAGCAGCAAGA1391     AspAspValLysIleIleAsnSerAspAsnValGlnGluAlaAlaArg     450455460     GAAACAGACGGATACTTCATAAAGAGCGGAATAGTCACTATAATCAAG1439     GluThrAspGlyTyrPheIleLysSerGlyIleValThrIleIleLys     465470475     GACGCCATGATTCCAAGTGGAACTGTAATCTAGAAATGGAGCATATAATAAAT1492     AspAlaMetIleProSerGlyThrValIle*     480485490     ATCACTGCCTATTTACAGTACCTATCTGAGTCTCCCACCATGACCCTTTGATTCAATCTT1552     TTAGTTATGTAAATATTTTTGGCTTTTGCGATTTTGCCATAAATTTGAAGAAGCGAGGAT1612     TCAGGGACGATAGTGCTATGAATTGGAAGAAAGGATTTGGGGGATATCTTTGTAAAGACA1672     TTTTGACTACTGGGCACTAAAAATTTGGTAATGCTATACCAAAATATATAAAAAGATCTT1732     GCTGGGTTTTGGTAAAAAAAAAAAAAAAAAA1763     (2) INFORMATION FOR SEQ ID NO:4:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 489 amino acids     (B) TYPE: amino acid     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: protein     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:     IleThrValProSerThrSerSerLysAsnLeuGlnAsnSerLeuAla     151015     PheSerSerSerSerLeuSerGlyAspLysIleGlnThrThrSerPhe     202530     LeuAsnArgArgTyrCysArgIleSerSerArgAlaProIleValVal     354045     SerProLysAlaValSerAspSerLysAsnSerGlnThrCysLeuAsp     505560     ProGluAlaSerArgSerValLeuGlyIleIleLeuGlyGlyGlyAla     65707580     GlyThrArgLeuTyrProLeuThrLysLysArgAlaLysProAlaVal     859095     ProLeuGlyAlaAsnTyrArgLeuIleAspIleProValSerAsnCys     100105110     LeuAsnSerAsnIleSerLysIleTyrValLeuThrGlnPheAsnSer     115120125     AlaSerLeuAsnArgHisLeuSerArgAlaTyrAlaSerAsnMetGly     130135140     GlyTyrLysAsnGluGlyPheValGluValLeuAlaAlaGlnGlnSer     145150155160     ProGluAsnProAsnTrpPheGlnGlyThrAlaAspAlaValArgGln     165170175     TyrLeuTrpLeuPheGluGluHisAsnValLeuGluTyrLeuIleLeu     180185190     AlaGlyAspHisLeuTyrArgMetAspTyrGluArgPheValGlnAla     195200205     HisArgGluThrAspAlaAspIleThrValAlaAlaLeuProMetAsp     210215220     GluLysArgAlaThrAlaPheGlyLeuMetLysIleAspGluGluGly     225230235240     ArgIleIleGluPheAlaGluLysProLysGlyGluGlnLeuLysAla     245250255     MetLysValAspThrThrIleLeuGlyLeuAspAspGluArgAlaLys     260265270     GluMetProPheIleAlaSerMetGlyIleTyrValIleSerLysAsp     275280285     ValMetLeuAsnLeuLeuArgGluGlnPheProGlyAlaAsnAspPhe     290295300     GlySerGluValIleProGlyAlaThrSerIleGlyLeuArgValGln     305310315320     AlaTyrLeuTyrAspGlyTyrTrpGluAspIleGlyThrIleGluAla     325330335     PheTyrAsnAlaAsnLeuGlyIleThrLysLysProValProAspPhe     340345350     SerPheTyrAspArgSerSerProIleTyrThrGlnProArgTyrLeu     355360365     ProProSerLysMetLeuAspAlaAspIleThrAspSerValIleGly     370375380     GluGlyCysValIleLysAsnCysLysIleHisHisSerValIleGly     385390395400     LeuArgSerCysIleSerGluGlyAlaIleIleGluAspThrLeuLeu     405410415     MetGlyAlaAspTyrTyrGluThrAspAlaAspArgLysPheLeuAla     420425430     AlaLysGlySerValProIleGlyIleGlyAsnAlaArgIleGlyAsp     435440445     AspValLysIleIleAsnSerAspAsnValGlnGluAlaAlaArgGlu     450455460     ThrAspGlyTyrPheIleLysSerGlyIleValThrIleIleLysAsp     465470475480     AlaMetIleProSerGlyThrValIle     485     (2) INFORMATION FOR SEQ ID NO:5:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 3635 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: cDNA     (vi) ORIGINAL SOURCE:     (A) ORGANISM: Beta vulgaris     (C) INDIVIDUAL ISOLATE: Saccharosephosphate Synthase     (vii) IMMEDIATE SOURCE:     (A) LIBRARY: phage lamda zap     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 30..3167     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:     GGGCTGCAGGGAAGCTCTGAACTTCAAAAATGGCGGGAAATGATTGGATAAAC53     MetAlaGlyAsnAspTrpIleAsn     15     AGTTATTTAGAGGCAATTCTGGATGTGGGTCCAGGACTTGATGATGCA101     SerTyrLeuGluAlaIleLeuAspValGlyProGlyLeuAspAspAla     101520     AAATCATCTTTGCTTTTGAGAGAAAGAGGCAGGTTTAGTCCTACTCGT149     LysSerSerLeuLeuLeuArgGluArgGlyArgPheSerProThrArg     25303540     TACTTTGTTGAAGAAGTTATCACTGGTTTTGATGAAACCGACCTTCAT197     TyrPheValGluGluValIleThrGlyPheAspGluThrAspLeuHis     455055     CGTTCATGGGTTCGGGCACAAGCAACAAGGAGTCCTCAAGAGAGGAAT245     ArgSerTrpValArgAlaGlnAlaThrArgSerProGlnGluArgAsn     606570     ACTAGATTGGAGAACATGTGTTGGAGAATTTGGAATTTGGCTCGTCAG293     ThrArgLeuGluAsnMetCysTrpArgIleTrpAsnLeuAlaArgGln     758085     AAGAAGCAGCTTGAGAATGAAGAAGCTCAGCGGAAGACAAAACGTCGT341     LysLysGlnLeuGluAsnGluGluAlaGlnArgLysThrLysArgArg     9095100     ATGGAGCTTGAGAGGGGTCGTCGAGAAGCAACTGCTGATATGTCGGAG389     MetGluLeuGluArgGlyArgArgGluAlaThrAlaAspMetSerGlu     105110115120     GACTTATCAGAAGGCGAAAAGGACATTTCAGCTCATGGTGATAGCACC437     AspLeuSerGluGlyGluLysAspIleSerAlaHisGlyAspSerThr     125130135     CGTCCTAGATTGCCAAGAATAAATTCTCTTGATGCTATGGAGACATGG485     ArgProArgLeuProArgIleAsnSerLeuAspAlaMetGluThrTrp     140145150     ATTAGTCAACAAAAGGAAAAAAAACTCTACCTTGTTTTGATAAGTCTT533     IleSerGlnGlnLysGluLysLysLeuTyrLeuValLeuIleSerLeu     155160165     CATGGTTTGATACGAGGTGAAAACATGGAACTTGGCCGTGATTCTGAT581     HisGlyLeuIleArgGlyGluAsnMetGluLeuGlyArgAspSerAsp     170175180     ACTGGTGGTCAGGTTAAGTATGTGGTTGAGCTTGCAAGGGCTCTAGGT629     ThrGlyGlyGlnValLysTyrValValGluLeuAlaArgAlaLeuGly     185190195200     TCGATGCCAGGTGTTTATAGAGTTGATTTGCTAACTAGGCAAGTTTCA677     SerMetProGlyValTyrArgValAspLeuLeuThrArgGlnValSer     205210215     TCTCCTGACGTGGATTGGAGTTATGGGGAGCCTACTGAGATGCTGAAT725     SerProAspValAspTrpSerTyrGlyGluProThrGluMetLeuAsn     220225230     CCAAGGGATTCCAATGGTTTTGATGATGATGATGATGAAATGGGAGAG773     ProArgAspSerAsnGlyPheAspAspAspAspAspGluMetGlyGlu     235240245     AGTAGTGGTGCTTACATTGTTCGTATACCATTTGGGCCGAGGGATAAG821     SerSerGlyAlaTyrIleValArgIleProPheGlyProArgAspLys     250255260     TATATCGCAAAAGAAGAGCTTTGGCCCTATATTCCTGAATTTGTTGAT869     TyrIleAlaLysGluGluLeuTrpProTyrIleProGluPheValAsp     265270275280     GGTGCTCTAAACCACATAGTTCAAATGTCCAAAGTTTTAGGTGAGCAA917     GlyAlaLeuAsnHisIleValGlnMetSerLysValLeuGlyGluGln     285290295     ATTGGTAGCGGGGAAACAGTTTGGCCAGTTGCCATTCATGGACATTAT965     IleGlySerGlyGluThrValTrpProValAlaIleHisGlyHisTyr     300305310     GCTGATGCTGGTGATTCTGCTGCTCTTCTTTCTGGTGGCCTAAATGTT1013     AlaAspAlaGlyAspSerAlaAlaLeuLeuSerGlyGlyLeuAsnVal     315320325     CCAATGCTTTTAACGGGGCATTCTCTTGGCCGAGACAAGTTAGAGCAG1061     ProMetLeuLeuThrGlyHisSerLeuGlyArgAspLysLeuGluGln     330335340     CTCCTCAAACAGGGTCGAATGTCTAAAGATGACATAAACAATACATAC1109     LeuLeuLysGlnGlyArgMetSerLysAspAspIleAsnAsnThrTyr     345350355360     AAAATAATGCGTAGGATAGAAGCCGAAGAGTTATCACTTGATGCCTCT1157     LysIleMetArgArgIleGluAlaGluGluLeuSerLeuAspAlaSer     365370375     GAGATAGTCATAACTAGTACAAGACAAGAAATAGAAGAGCAATGGCAC1205     GluIleValIleThrSerThrArgGlnGluIleGluGluGlnTrpHis     380385390     CTCTATGATGGGTTTGATCCTGTGCTAGAACGTAAACTCCGTGCTAGG1253     LeuTyrAspGlyPheAspProValLeuGluArgLysLeuArgAlaArg     395400405     ATGAAGCGTGGTGTAAGCTGTTATGGAAGGTTCATGCCCCGGATGGTT1301     MetLysArgGlyValSerCysTyrGlyArgPheMetProArgMetVal     410415420     GTTATTCCTCCTGGAATGGAATTCAATCATATTGTTCCACATGAGGGT1349     ValIleProProGlyMetGluPheAsnHisIleValProHisGluGly     425430435440     GATATGGATGGTGAAACAGAAGAAACTGAAGAGCATCCTACATCACCT1397     AspMetAspGlyGluThrGluGluThrGluGluHisProThrSerPro     445450455     GATCCACCTATCTGGGCTGAGATTATGCGCTTCTTTTCTAAACCAAGG1445     AspProProIleTrpAlaGluIleMetArgPhePheSerLysProArg     460465470     AAGCCAATGATACTTGCCCTTGCTAGGCCTGACCCGAAGAAGAATATC1493     LysProMetIleLeuAlaLeuAlaArgProAspProLysLysAsnIle     475480485     ACGACTTTGGTCAAAGCATTTGGAGAATGCCGTCCACTAAGGGAGCTA1541     ThrThrLeuValLysAlaPheGlyGluCysArgProLeuArgGluLeu     490495500     GCTAATCTTACTCTTATAATGGGTAACCGAGATGGTATTGACGAGATG1589     AlaAsnLeuThrLeuIleMetGlyAsnArgAspGlyIleAspGluMet     505510515520     TCAAGCACCAGTTCTTCAGTTCTCCTGTCAGTGCTTAAGCTAATTGAT1637     SerSerThrSerSerSerValLeuLeuSerValLeuLysLeuIleAsp     525530535     CAATACGACCTTTATGGTCAAGTAGCATACCCCAAACATCACAAGCAA1685     GlnTyrAspLeuTyrGlyGlnValAlaTyrProLysHisHisLysGln     540545550     GCTGATGTTCCTGAGATTTATCGTTTGGCAGCAAAGACAAAGGGAGTC1733     AlaAspValProGluIleTyrArgLeuAlaAlaLysThrLysGlyVal     555560565     TTTATTAATCCAGCTTTTATTGAGCCATTTGGGCTGACTCTAATAGAG1781     PheIleAsnProAlaPheIleGluProPheGlyLeuThrLeuIleGlu     570575580     GCAGCAGCTCATGGTTTACCGATGGTTGCTACGAAAAATGGAGGCCCT1829     AlaAlaAlaHisGlyLeuProMetValAlaThrLysAsnGlyGlyPro     585590595600     GTTGATATCCAGAGGGTCCTTGATAATGGTCTTCTTGTGGATCCTCAT1877     ValAspIleGlnArgValLeuAspAsnGlyLeuLeuValAspProHis     605610615     GAGCAGCAGTCTATTGCTACTGCTTTGCTGAAGCTTGTTGCTGATAAG1925     GluGlnGlnSerIleAlaThrAlaLeuLeuLysLeuValAlaAspLys     620625630     CAACTATGGACAAAATGCCAGCAAAATGGACTGAAAAATATTCATCTC1973     GlnLeuTrpThrLysCysGlnGlnAsnGlyLeuLysAsnIleHisLeu     635640645     TACTCTTGGCCAGAGCATTCGAAGACATACCTATCTCGAATAGCCAGT2021     TyrSerTrpProGluHisSerLysThrTyrLeuSerArgIleAlaSer     650655660     TCGAGACAAAGGCAACCACAGTGGCAAAGAAGTAGTGATGAAGGGCTT2069     SerArgGlnArgGlnProGlnTrpGlnArgSerSerAspGluGlyLeu     665670675680     GACAATCAAGAGCCTGAATCTCCAAGTGATTCTTTAAGAGATATAAAG2117     AspAsnGlnGluProGluSerProSerAspSerLeuArgAspIleLys     685690695     GATATATCTCTAAACCTTGAAGTTCTCGTTAGACCGGAGAAAAGGGTG2165     AspIleSerLeuAsnLeuGluValLeuValArgProGluLysArgVal     700705710     AAGACGTTGAAAATCTTGGGATTGATGACAAAAGCAAATTCGAGAATG2213     LysThrLeuLysIleLeuGlyLeuMetThrLysAlaAsnSerArgMet     715720725     CTGTTATGTTCATGGTCTAATGGTGTCCATAAGATGCTTCGGAAGGCT2261     LeuLeuCysSerTrpSerAsnGlyValHisLysMetLeuArgLysAla     730735740     CGGTTCTCTGACAAAGTAGATCAGGCTTCTAGTAAATATCCAGCATTT2309     ArgPheSerAspLysValAspGlnAlaSerSerLysTyrProAlaPhe     745750755760     AGGAGGAGAAAACTTATATATGTTATTGCTGTAGACGGGGATTATGAA2357     ArgArgArgLysLeuIleTyrValIleAlaValAspGlyAspTyrGlu     765770775     GATGGACTTTTTGATATTGTTCGGAGGATATTTGATGCTGCTGGCAAG2405     AspGlyLeuPheAspIleValArgArgIlePheAspAlaAlaGlyLys     780785790     GAGAAGATTGAAGGTTCCATCGGGTTTATATTGTCAACATCCTATTCT2453     GluLysIleGluGlySerIleGlyPheIleLeuSerThrSerTyrSer     795800805     ATGCCCGAAATTCAGAACTATTTGCTATCAAAAGGCTTCAATCTTCAT2501     MetProGluIleGlnAsnTyrLeuLeuSerLysGlyPheAsnLeuHis     810815820     GATTTTGATGCATATATATGCAACAGTGGGAGTGAGTTGTACTATTCA2549     AspPheAspAlaTyrIleCysAsnSerGlySerGluLeuTyrTyrSer     825830835840     TCTTTGAACTCAGAGGAGAGTAATATTATAGCAGATTCAGATTACCAT2597     SerLeuAsnSerGluGluSerAsnIleIleAlaAspSerAspTyrHis     845850855     TCACACATAGAGTACAGATGGGGTGGAGAAGGCCTTAGAAGGACTTTG2645     SerHisIleGluTyrArgTrpGlyGlyGluGlyLeuArgArgThrLeu     860865870     CTTCGCTGGGCAGCTTCCATCACAGAAAAAAATGGTGAAAACGAAGAA2693     LeuArgTrpAlaAlaSerIleThrGluLysAsnGlyGluAsnGluGlu     875880885     CAGGTTATTACTGAAGATGAAGAAGTTTCTACGGGTTATTGCTTTGCG2741     GlnValIleThrGluAspGluGluValSerThrGlyTyrCysPheAla     890895900     TTTAAAATAAAGAACCAAAATAAGGTTCCCCCTACGAAGGAGCTCCGC2789     PheLysIleLysAsnGlnAsnLysValProProThrLysGluLeuArg     905910915920     AAGTCAATGAGGATTCAAGCTCTTCGTTGCCATGTGATTTACTGTCAG2837     LysSerMetArgIleGlnAlaLeuArgCysHisValIleTyrCysGln     925930935     AACGGATCTAAAATGAATGTGATTCCAGTACTAGCATCCCGTTCTCAA2885     AsnGlySerLysMetAsnValIleProValLeuAlaSerArgSerGln     940945950     GCCCTCAGGTATCTTTATGTTCGTTGGGGAGTTGAGTTGTCGAAGATG2933     AlaLeuArgTyrLeuTyrValArgTrpGlyValGluLeuSerLysMet     955960965     GTTGTCTTTGTTGGAGAATGTGGTGACACAGATTATGAAGGCTTGCTT2981     ValValPheValGlyGluCysGlyAspThrAspTyrGluGlyLeuLeu     970975980     GGCGGGGTCCATAAAACCGTAATACTGAAGGGAGTCTCCAACACTGCT3029     GlyGlyValHisLysThrValIleLeuLysGlyValSerAsnThrAla     9859909951000     TTAAGGTCTCTCCATGCCAACAGAAGTTACCCTCTTTCACATGTCGTG3077     LeuArgSerLeuHisAlaAsnArgSerTyrProLeuSerHisValVal     100510101015     TCGCTTGACAGCCCCAATATTGGCGAGGTGAGCAAAGGGTGCAGCAGC3125     SerLeuAspSerProAsnIleGlyGluValSerLysGlyCysSerSer     102010251030     TCCGAGATCCAGTCCATCGTCACAAAACTCTCCAAAGCTTAA3167     SerGluIleGlnSerIleValThrLysLeuSerLysAla*     103510401045     TCAGATATCTGCTGCTTTCTTTTGGGTAAGCAAGGTTTCATCTTATATGATTATATCATA3227     AGATACTATATAAGCACCTTATTGGTAAGTCAGTCCCATAATAATAATGTACTTCAGAAC3287     CACAATACTTAAAAGTTGGTTCAGTAGTGATTAGTCTCATAATAATCATATAATTACACA3347     TCCGCTGTTAACTAGTGGTAATATCTAAGCTCAACAATAAAGATGTAAAATGCTAGTATG3407     GAAATGAATTGCTAGCTGTTGATCTCTTTCCCTTTATTCTGTATTATTTCTTTCCTCATC3467     TCATGTAAAAACAATTTTCTGAAGGTGTACAGTTTTTTCCCCTTATATATCTGTATTATT3527     TCTACTATTTTTTGTTTGTAAGAATATCCTCTCATCGAGGAGTGATAATTAAATAACCGG3587     CTTGCTAAATATAAAGCTTATTCGAGTTAAAAAAAAAAAAAAAAAAAA3635     (2) INFORMATION FOR SEQ ID NO:6:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 1045 amino acids     (B) TYPE: amino acid     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: protein     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:     MetAlaGlyAsnAspTrpIleAsnSerTyrLeuGluAlaIleLeuAsp     151015     ValGlyProGlyLeuAspAspAlaLysSerSerLeuLeuLeuArgGlu     202530     ArgGlyArgPheSerProThrArgTyrPheValGluGluValIleThr     354045     GlyPheAspGluThrAspLeuHisArgSerTrpValArgAlaGlnAla     505560     ThrArgSerProGlnGluArgAsnThrArgLeuGluAsnMetCysTrp     65707580     ArgIleTrpAsnLeuAlaArgGlnLysLysGlnLeuGluAsnGluGlu     859095     AlaGlnArgLysThrLysArgArgMetGluLeuGluArgGlyArgArg     100105110     GluAlaThrAlaAspMetSerGluAspLeuSerGluGlyGluLysAsp     115120125     IleSerAlaHisGlyAspSerThrArgProArgLeuProArgIleAsn     130135140     SerLeuAspAlaMetGluThrTrpIleSerGlnGlnLysGluLysLys     145150155160     LeuTyrLeuValLeuIleSerLeuHisGlyLeuIleArgGlyGluAsn     165170175     MetGluLeuGlyArgAspSerAspThrGlyGlyGlnValLysTyrVal     180185190     ValGluLeuAlaArgAlaLeuGlySerMetProGlyValTyrArgVal     195200205     AspLeuLeuThrArgGlnValSerSerProAspValAspTrpSerTyr     210215220     GlyGluProThrGluMetLeuAsnProArgAspSerAsnGlyPheAsp     225230235240     AspAspAspAspGluMetGlyGluSerSerGlyAlaTyrIleValArg     245250255     IleProPheGlyProArgAspLysTyrIleAlaLysGluGluLeuTrp     260265270     ProTyrIleProGluPheValAspGlyAlaLeuAsnHisIleValGln     275280285     MetSerLysValLeuGlyGluGlnIleGlySerGlyGluThrValTrp     290295300     ProValAlaIleHisGlyHisTyrAlaAspAlaGlyAspSerAlaAla     305310315320     LeuLeuSerGlyGlyLeuAsnValProMetLeuLeuThrGlyHisSer     325330335     LeuGlyArgAspLysLeuGluGlnLeuLeuLysGlnGlyArgMetSer     340345350     LysAspAspIleAsnAsnThrTyrLysIleMetArgArgIleGluAla     355360365     GluGluLeuSerLeuAspAlaSerGluIleValIleThrSerThrArg     370375380     GlnGluIleGluGluGlnTrpHisLeuTyrAspGlyPheAspProVal     385390395400     LeuGluArgLysLeuArgAlaArgMetLysArgGlyValSerCysTyr     405410415     GlyArgPheMetProArgMetValValIleProProGlyMetGluPhe     420425430     AsnHisIleValProHisGluGlyAspMetAspGlyGluThrGluGlu     435440445     ThrGluGluHisProThrSerProAspProProIleTrpAlaGluIle     450455460     MetArgPhePheSerLysProArgLysProMetIleLeuAlaLeuAla     465470475480     ArgProAspProLysLysAsnIleThrThrLeuValLysAlaPheGly     485490495     GluCysArgProLeuArgGluLeuAlaAsnLeuThrLeuIleMetGly     500505510     AsnArgAspGlyIleAspGluMetSerSerThrSerSerSerValLeu     515520525     LeuSerValLeuLysLeuIleAspGlnTyrAspLeuTyrGlyGlnVal     530535540     AlaTyrProLysHisHisLysGlnAlaAspValProGluIleTyrArg     545550555560     LeuAlaAlaLysThrLysGlyValPheIleAsnProAlaPheIleGlu     565570575     ProPheGlyLeuThrLeuIleGluAlaAlaAlaHisGlyLeuProMet     580585590     ValAlaThrLysAsnGlyGlyProValAspIleGlnArgValLeuAsp     595600605     AsnGlyLeuLeuValAspProHisGluGlnGlnSerIleAlaThrAla     610615620     LeuLeuLysLeuValAlaAspLysGlnLeuTrpThrLysCysGlnGln     625630635640     AsnGlyLeuLysAsnIleHisLeuTyrSerTrpProGluHisSerLys     645650655     ThrTyrLeuSerArgIleAlaSerSerArgGlnArgGlnProGlnTrp     660665670     GlnArgSerSerAspGluGlyLeuAspAsnGlnGluProGluSerPro     675680685     SerAspSerLeuArgAspIleLysAspIleSerLeuAsnLeuGluVal     690695700     LeuValArgProGluLysArgValLysThrLeuLysIleLeuGlyLeu     705710715720     MetThrLysAlaAsnSerArgMetLeuLeuCysSerTrpSerAsnGly     725730735     ValHisLysMetLeuArgLysAlaArgPheSerAspLysValAspGln     740745750     AlaSerSerLysTyrProAlaPheArgArgArgLysLeuIleTyrVal     755760765     IleAlaValAspGlyAspTyrGluAspGlyLeuPheAspIleValArg     770775780     ArgIlePheAspAlaAlaGlyLysGluLysIleGluGlySerIleGly     785790795800     PheIleLeuSerThrSerTyrSerMetProGluIleGlnAsnTyrLeu     805810815     LeuSerLysGlyPheAsnLeuHisAspPheAspAlaTyrIleCysAsn     820825830     SerGlySerGluLeuTyrTyrSerSerLeuAsnSerGluGluSerAsn     835840845     IleIleAlaAspSerAspTyrHisSerHisIleGluTyrArgTrpGly     850855860     GlyGluGlyLeuArgArgThrLeuLeuArgTrpAlaAlaSerIleThr     865870875880     GluLysAsnGlyGluAsnGluGluGlnValIleThrGluAspGluGlu     885890895     ValSerThrGlyTyrCysPheAlaPheLysIleLysAsnGlnAsnLys     900905910     ValProProThrLysGluLeuArgLysSerMetArgIleGlnAlaLeu     915920925     ArgCysHisValIleTyrCysGlnAsnGlySerLysMetAsnValIle     930935940     ProValLeuAlaSerArgSerGlnAlaLeuArgTyrLeuTyrValArg     945950955960     TrpGlyValGluLeuSerLysMetValValPheValGlyGluCysGly     965970975     AspThrAspTyrGluGlyLeuLeuGlyGlyValHisLysThrValIle     980985990     LeuLysGlyValSerAsnThrAlaLeuArgSerLeuHisAlaAsnArg     99510001005     SerTyrProLeuSerHisValValSerLeuAspSerProAsnIleGly     101010151020     GluValSerLysGlyCysSerSerSerGluIleGlnSerIleValThr     1025103010351040     LysLeuSerLysAla     1045     (2) INFORMATION FOR SEQ ID NO:7:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 2563 base pairs     (B) TYPE: nucleic acid     (C) STRANDEDNESS: single     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: cDNA     (vi) ORIGINAL SOURCE:     (A) ORGANISM: Beta vulgaris     (C) INDIVIDUAL ISOLATE: Saccharosesynthase     (vii) IMMEDIATE SOURCE:     (A) LIBRARY: phage lamda zap     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 3..2303     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:     CTGCAGGAGGGAAACAAATTCTTAGCGATGGCCCGTTTAGCGAAGTT47     AlaGlyGlyLysGlnIleLeuSerAspGlyProPheSerGluVal     151015     CTTAGGTCTGCTCAGGAAGCAATAGTTGTTCCTCCCTTTGTTGCTATA95     LeuArgSerAlaGlnGluAlaIleValValProProPheValAlaIle     202530     GCAGTCCGTCCAAGACCTGGAGTTTGGGAATATGTTCGTGTTAATGTC143     AlaValArgProArgProGlyValTrpGluTyrValArgValAsnVal     354045     TCTGAACTGAATGTGGAGCAGCTAACTGTGTCTGAGTATCTCCATTTC191     SerGluLeuAsnValGluGlnLeuThrValSerGluTyrLeuHisPhe     505560     AAGGAAGAACTTGTGGATGGAAAGGCTGATGACCACTATGTGCTTGAG239     LysGluGluLeuValAspGlyLysAlaAspAspHisTyrValLeuGlu     657075     CTTGATTTCGAGCCTTTTAATGAATCAGTTCCACGTCCAACTCGCTCT287     LeuAspPheGluProPheAsnGluSerValProArgProThrArgSer     80859095     TCATCAATTGGTAATGGTGTTCAGTTCCTCAATCGGCACCTGTCATCA335     SerSerIleGlyAsnGlyValGlnPheLeuAsnArgHisLeuSerSer     100105110     AGCATGTTCTGCAACAAAGATTGCTTGGAGCCGTTACTTGATTTTCTT383     SerMetPheCysAsnLysAspCysLeuGluProLeuLeuAspPheLeu     115120125     AGAGTGCACAAACATAAAGGAGTTGTCATGATGTTGAATGATCGGATA431     ArgValHisLysHisLysGlyValValMetMetLeuAsnAspArgIle     130135140     CAGACTATCCAGCGTCTTCAGTCTGCATTGTCTAAAGCTGAGGATTAT479     GlnThrIleGlnArgLeuGlnSerAlaLeuSerLysAlaGluAspTyr     145150155     CTTATCAAACTTCCAGCAGATACACCTTACTCTGAGTTCGAATTTGTA527     LeuIleLysLeuProAlaAspThrProTyrSerGluPheGluPheVal     160165170175     ATCCAAGGTATGGGTTTTGAAAGAGGCTGGGGTGATACTGCTGAAAGG575     IleGlnGlyMetGlyPheGluArgGlyTrpGlyAspThrAlaGluArg     180185190     GTTCTAGAAATGATGCATCTACTACTAGATATCCTTCAGGCTCCCGAT623     ValLeuGluMetMetHisLeuLeuLeuAspIleLeuGlnAlaProAsp     195200205     CCGTCTACATTAGAGACATTTCTGGGAAGACTTCCCATGGTGTTTAAT671     ProSerThrLeuGluThrPheLeuGlyArgLeuProMetValPheAsn     210215220     GTGGTCATTTTGTCTGTACATGGATATTTTGGACAGGCACATGTGCTC719     ValValIleLeuSerValHisGlyTyrPheGlyGlnAlaHisValLeu     225230235     GGCTTGCCTGACACTGGTGGGCAGATAGTTTATATACTTGACCAAGTG767     GlyLeuProAspThrGlyGlyGlnIleValTyrIleLeuAspGlnVal     240245250255     CGGTCTCTGGAACATGAAATGCTCCAACGAATAAAGAAGCAAGGACTA815     ArgSerLeuGluHisGluMetLeuGlnArgIleLysLysGlnGlyLeu     260265270     GATGTGACTCCTAGAATTCTTATCGTGAGTCGGTTGATTCCTGACGCT863     AspValThrProArgIleLeuIleValSerArgLeuIleProAspAla     275280285     AAAGGGACCACGTGCAATCAACGTATGGAGAAAGTCAGTGGAACAGAG911     LysGlyThrThrCysAsnGlnArgMetGluLysValSerGlyThrGlu     290295300     CATGCTAGTATCCTGAGAGTTCCTTTCCGATCAGAGAAAGGAATCCTC959     HisAlaSerIleLeuArgValProPheArgSerGluLysGlyIleLeu     305310315     CGCAAATGGATATCTAGATTTGATGTATGGCCTTATTTAGAGACCTTC1007     ArgLysTrpIleSerArgPheAspValTrpProTyrLeuGluThrPhe     320325330335     ACTGAGGATGCAGCTGGTGAAATTATTGGCGAGTTGCAGGGTCGTCCA1055     ThrGluAspAlaAlaGlyGluIleIleGlyGluLeuGlnGlyArgPro     340345350     GATCTGATAATTGGCAACTACAGCGATGGGAATATAGTTGCTTCTTTA1103     AspLeuIleIleGlyAsnTyrSerAspGlyAsnIleValAlaSerLeu     355360365     TTGTCCCACAAAATGGGTGTCACCCAGTGCAATATAGCCCATGCATTG1151     LeuSerHisLysMetGlyValThrGlnCysAsnIleAlaHisAlaLeu     370375380     GAGAAAACCAAGTATCCAGATTCTGATATTTACTGGAAAAGATTTGAG1199     GluLysThrLysTyrProAspSerAspIleTyrTrpLysArgPheGlu     385390395     GACAAATATCACTTCTCGTGTCAATTTTCAGCTGACTTGATGGCAATG1247     AspLysTyrHisPheSerCysGlnPheSerAlaAspLeuMetAlaMet     400405410415     AATCATGCTGATTTCATCATTACGAGTACTTACCAAGAGATAGCTGGA1295     AsnHisAlaAspPheIleIleThrSerThrTyrGlnGluIleAlaGly     420425430     ACGAAGAATACTGTTGGTCAATATGAAAGCCATAAGGCCTTTACTTTT1343     ThrLysAsnThrValGlyGlnTyrGluSerHisLysAlaPheThrPhe     435440445     CCGGGGCTGTATCGGGTGGTTCACGGGATTGATGTCTTTGATCCCAAG1391     ProGlyLeuTyrArgValValHisGlyIleAspValPheAspProLys     450455460     TTTAATATTGTCTCGCCAGGGGCAGACATGGCCATCTACTTCCCATTT1439     PheAsnIleValSerProGlyAlaAspMetAlaIleTyrPheProPhe     465470475     TCAGAGAAGGATGTCACCTGTCTCACTTCACTTCATAGACTTATAGAG1487     SerGluLysAspValThrCysLeuThrSerLeuHisArgLeuIleGlu     480485490495     CAGCTCCTATTCAAACCTGAGCAGAACGAAGAACACATTGGTGTATTA1535     GlnLeuLeuPheLysProGluGlnAsnGluGluHisIleGlyValLeu     500505510     GATGATACCTCAAAGCCAATTATATTTTCCATGGCGAGGCTAGACCGT1583     AspAspThrSerLysProIleIlePheSerMetAlaArgLeuAspArg     515520525     GTGAAGAATATAACAGGGCTGGTAGAGTGCTATGGCAAGAATGCGAAA1631     ValLysAsnIleThrGlyLeuValGluCysTyrGlyLysAsnAlaLys     530535540     CTCAGGGAACTGGCAAACCTGGTTGTAGTGGCTGGGTACAATGATGTA1679     LeuArgGluLeuAlaAsnLeuValValValAlaGlyTyrAsnAspVal     545550555     AAAAAGTCGAATGACAGGGAGGAAATTGCCGAAATCGAGAAGATGCAC1727     LysLysSerAsnAspArgGluGluIleAlaGluIleGluLysMetHis     560565570575     AGGCTTATACAGGAGTATAATTTAAGAGGACAATTTCGCTGGATTGCT1775     ArgLeuIleGlnGluTyrAsnLeuArgGlyGlnPheArgTrpIleAla     580585590     TCTCAAACAAATAGAGTACGAAATGGTGAACTCTATCGCTACATTTGT1823     SerGlnThrAsnArgValArgAsnGlyGluLeuTyrArgTyrIleCys     595600605     GACAAAGGAGGTATTTTTGCGCAGCCTGCATTTTATGAAGCATTTGGG1871     AspLysGlyGlyIlePheAlaGlnProAlaPheTyrGluAlaPheGly     610615620     CTTACAGTTGTTGAAGCCATGACCTGTGGTCTTCCCACATTTGCTACC1919     LeuThrValValGluAlaMetThrCysGlyLeuProThrPheAlaThr     625630635     TGCCACGGTGGTCCAGCTGAGATTATAGAAGACGGTGTTTCAGGATTT1967     CysHisGlyGlyProAlaGluIleIleGluAspGlyValSerGlyPhe     640645650655     CATATCGATCCATATCATGCTGATCAGGCAGAAAAAATGACTGAATTC2015     HisIleAspProTyrHisAlaAspGlnAlaGluLysMetThrGluPhe     660665670     TTTGTCAAGTGCAGAGAGGATCCAAACTACTGGACTAAAATCTCTGCA2063     PheValLysCysArgGluAspProAsnTyrTrpThrLysIleSerAla     675680685     GGAGGGTTACTAAGGATCAAAGAAAGATATACCTGGCAAAAGTATTCT2111     GlyGlyLeuLeuArgIleLysGluArgTyrThrTrpGlnLysTyrSer     690695700     GAAAGGTTAATGACATTGGCAGGGGTGTATGGTTTCTGGAAATATGTC2159     GluArgLeuMetThrLeuAlaGlyValTyrGlyPheTrpLysTyrVal     705710715     TCTAAACTAGAGAGAAGAGAGACACGACGTTATCTTGAGATGTTCTAC2207     SerLysLeuGluArgArgGluThrArgArgTyrLeuGluMetPheTyr     720725730735     ATTTTGAAGTTCCGTGATCTGGCCAACTCTGTTCCGCTGGCAACAGAT2255     IleLeuLysPheArgAspLeuAlaAsnSerValProLeuAlaThrAsp     740745750     GAAGAGCCTTCTACTACTGATGCAGTTGCGACATTCCGTGGACCTTGA2303     GluGluProSerThrThrAspAlaValAlaThrPheArgGlyPro*     755760765     ACGCTGCTGCTTACTGAGGTTCCAAGTTGTGTATATATTACTGTGAAAGGAATAAGTGTA2363     GCTACACAAAAGGTTCTCAACTATTAGTATCTTCTCTGTGTAAATAACGAGAGTGAAAAA2423     TGTAATATTGTTGATGTCTTGAAAACTGAGTTTGCTTTGTTTATTTTTAAGTGTATGACA2483     ATATGTATCATATAACGGATTCTTCAGTGATCATATCAAAAACTACTGACCATCGAAGTT2543     AATGAAAATCGACAGCAACA2563     (2) INFORMATION FOR SEQ ID NO:8:     (i) SEQUENCE CHARACTERISTICS:     (A) LENGTH: 766 amino acids     (B) TYPE: amino acid     (D) TOPOLOGY: linear     (ii) MOLECULE TYPE: protein     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:     AlaGlyGlyLysGlnIleLeuSerAspGlyProPheSerGluValLeu     151015     ArgSerAlaGlnGluAlaIleValValProProPheValAlaIleAla     202530     ValArgProArgProGlyValTrpGluTyrValArgValAsnValSer     354045     GluLeuAsnValGluGlnLeuThrValSerGluTyrLeuHisPheLys     505560     GluGluLeuValAspGlyLysAlaAspAspHisTyrValLeuGluLeu     65707580     AspPheGluProPheAsnGluSerValProArgProThrArgSerSer     859095     SerIleGlyAsnGlyValGlnPheLeuAsnArgHisLeuSerSerSer     100105110     MetPheCysAsnLysAspCysLeuGluProLeuLeuAspPheLeuArg     115120125     ValHisLysHisLysGlyValValMetMetLeuAsnAspArgIleGln     130135140     ThrIleGlnArgLeuGlnSerAlaLeuSerLysAlaGluAspTyrLeu     145150155160     IleLysLeuProAlaAspThrProTyrSerGluPheGluPheValIle     165170175     GlnGlyMetGlyPheGluArgGlyTrpGlyAspThrAlaGluArgVal     180185190     LeuGluMetMetHisLeuLeuLeuAspIleLeuGlnAlaProAspPro     195200205     SerThrLeuGluThrPheLeuGlyArgLeuProMetValPheAsnVal     210215220     ValIleLeuSerValHisGlyTyrPheGlyGlnAlaHisValLeuGly     225230235240     LeuProAspThrGlyGlyGlnIleValTyrIleLeuAspGlnValArg     245250255     SerLeuGluHisGluMetLeuGlnArgIleLysLysGlnGlyLeuAsp     260265270     ValThrProArgIleLeuIleValSerArgLeuIleProAspAlaLys     275280285     GlyThrThrCysAsnGlnArgMetGluLysValSerGlyThrGluHis     290295300     AlaSerIleLeuArgValProPheArgSerGluLysGlyIleLeuArg     305310315320     LysTrpIleSerArgPheAspValTrpProTyrLeuGluThrPheThr     325330335     GluAspAlaAlaGlyGluIleIleGlyGluLeuGlnGlyArgProAsp     340345350     LeuIleIleGlyAsnTyrSerAspGlyAsnIleValAlaSerLeuLeu     355360365     SerHisLysMetGlyValThrGlnCysAsnIleAlaHisAlaLeuGlu     370375380     LysThrLysTyrProAspSerAspIleTyrTrpLysArgPheGluAsp     385390395400     LysTyrHisPheSerCysGlnPheSerAlaAspLeuMetAlaMetAsn     405410415     HisAlaAspPheIleIleThrSerThrTyrGlnGluIleAlaGlyThr     420425430     LysAsnThrValGlyGlnTyrGluSerHisLysAlaPheThrPhePro     435440445     GlyLeuTyrArgValValHisGlyIleAspValPheAspProLysPhe     450455460     AsnIleValSerProGlyAlaAspMetAlaIleTyrPheProPheSer     465470475480     GluLysAspValThrCysLeuThrSerLeuHisArgLeuIleGluGln     485490495     LeuLeuPheLysProGluGlnAsnGluGluHisIleGlyValLeuAsp     500505510     AspThrSerLysProIleIlePheSerMetAlaArgLeuAspArgVal     515520525     LysAsnIleThrGlyLeuValGluCysTyrGlyLysAsnAlaLysLeu     530535540     ArgGluLeuAlaAsnLeuValValValAlaGlyTyrAsnAspValLys     545550555560     LysSerAsnAspArgGluGluIleAlaGluIleGluLysMetHisArg     565570575     LeuIleGlnGluTyrAsnLeuArgGlyGlnPheArgTrpIleAlaSer     580585590     GlnThrAsnArgValArgAsnGlyGluLeuTyrArgTyrIleCysAsp     595600605     LysGlyGlyIlePheAlaGlnProAlaPheTyrGluAlaPheGlyLeu     610615620     ThrValValGluAlaMetThrCysGlyLeuProThrPheAlaThrCys     625630635640     HisGlyGlyProAlaGluIleIleGluAspGlyValSerGlyPheHis     645650655     IleAspProTyrHisAlaAspGlnAlaGluLysMetThrGluPhePhe     660665670     ValLysCysArgGluAspProAsnTyrTrpThrLysIleSerAlaGly     675680685     GlyLeuLeuArgIleLysGluArgTyrThrTrpGlnLysTyrSerGlu     690695700     ArgLeuMetThrLeuAlaGlyValTyrGlyPheTrpLysTyrValSer     705710715720     LysLeuGluArgArgGluThrArgArgTyrLeuGluMetPheTyrIle     725730735     LeuLysPheArgAspLeuAlaAsnSerValProLeuAlaThrAspGlu     740745750     GluProSerThrThrAspAlaValAlaThrPheArgGlyPro     755760765     __________________________________________________________________________ 

We claim:
 1. An isolated DNA molecule comprising a DNA sequence, wherein the sequence comprises Seq. ID No.
 1. 2. An isolated DNA molecule comprising a DNA sequence wherein the sequence comprises Seq. ID No.
 3. 3. An isolated DNA molecule comprising a DNA sequence wherein the sequence comprises Seq. ID No.
 5. 4. An isolated DNA molecule comprising a DNA sequence wherein the sequence comprises Seq. ID No.
 7. 5. An isolated DNA molecule selected from the group consisting of: (i) SEQ ID NO. 1; (ii) SEQ ID NO. 3; (iii) SEQ ID No. 5; (iv) SEQ ID NO. 7; and (v) SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO.
 7. 6. An isolated DNA molecule having a sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO.
 7. 7. An isolated DNA molecule having a nucleotide sequence comprising SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO.
 7. 8. A plasmid comprising at least one isolated DNA molecule as in any ot claims 1, 2, 3, 4, 5, 6, or
 7. 9. A plasmid comprising:a promoter sequence; at least one coding sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO, 7, wherein said coding sequence, upon expression produces a protein; and a termination sequence.
 10. A plasmid comprising:a promoter sequence; at least one coding sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7, wherein said coding sequence is in an anti-sense orientation with respect to the promoter; and a termination sequence.
 11. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises and expresses an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or
 7. 12. A method for producing a transformed plant comprising transforming a plant cell so that it comprises and expresses an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or 7 and regenerating the transformed plant from the plant cell.
 13. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or 7 in an anti-sense orientation.
 14. A method for producing a transformed plant comprising transforming a plant cell so that it comprises an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or 7 in an anti-sense orientation and regenerating the transformed plant from the plant cell.
 15. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises the plasmid of claim 8 and expresses the isolated DNA molecule therein.
 16. A method for producing a transformed plant comprising transforming a plant cell so that it comprises the plasmid of claim 8 and expresses the isolated DNA molecule therein, and regenerating the transformed plant from the plant cell.
 17. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises the plasmid of claim 8 and the isolated DNA molecule therein is in an anti-sense orientation.
 18. A method for producing a transformed plant comprising transforming a plant cell so that it comprises the plasmid of claim 8 and the isolated DNA molecule therein is in an anti-sense orientation, and regenerating the transformed plant from the plant cell.
 19. A method of producing a plant or plant cell comprising the steps of:producing a DNA molecule comprising the following sequences:(i) a promoter which is active in said plant or plant cell, (ii) a structural DNA sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7 positioned in a sense orientation; and transferring and incorporating said DNA molecule into the genome of a plant cell.
 20. A method of producing a plant or plant cell comprising the steps of:producing a DNA molecule comprising the following sequences:(i) a promoter which is active in said plant or plant cell, (ii) a structural DNA sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7 positioned in an anti-sense orientation; and transferring and incorporating said DNA molecule into the genome of a plant cell.
 21. A transformed plant cell comprising at least one isolated DNA molecule as in any of claims 1, 2, 3, 4, 5, 6, or
 7. 22. A transformed plant comprising at least one isolated DNA molecule as in any of claims 1, 2, 3, 4, 5, 6, or
 7. 23. A transgenic sugar beet plant comprising at least one isolated DNA molecule as in any of claims 1, 2, 3, 4, 5, 6, or
 7. 24. A plant or plant cell transformed with a plasmid according to claim
 9. 25. A plant or plant cell transformed with a plasmid according to claim
 10. 26. A transformed plant cell comprising the plasmid of claim
 8. 27. A transformed plant comprising the plant cell of claim
 21. 28. A transformed plant comprising the plant cell of claim
 26. 29. A transformed plant comprising the plasmid of claim
 8. 30. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO.
 1. 31. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO.
 3. 32. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO.
 5. 33. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO.
 7. 34. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEO ID NO.
 7. 35. A transformed plant cell comprising an isolated DNA molecule therein, and regenerating the plant from the plant cell having a nucleotide sequence selected from the group consisting of: SEQ ID. NO. 1; SEQ ID NO. 3; SEQ ID NO. 5; SEQ ID NO. 7; and SEQ ID. NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO.
 7. 